Unknown

Dataset Information

0

A method to culture human alveolar rhabdomyosarcoma cell lines as rhabdospheres demonstrates an enrichment in stemness and Notch signaling.


ABSTRACT: The development of three-dimensional cell culture techniques has allowed cancer researchers to study the stemness properties of cancer cells in in vitro culture. However, a method to grow PAX3-FOXO1 fusion-positive rhabdomyosarcoma (FP-RMS), an aggressive soft tissue sarcoma of childhood, has to date not been reported, hampering efforts to identify the dysregulated signaling pathways that underlie FP-RMS stemness. Here, we first examine the expression of canonical stem cell markers in human RMS tumors and cell lines. We then describe a method to grow FP-RMS cell lines as rhabdospheres and demonstrate that these spheres are enriched in expression of canonical stemness factors as well as Notch signaling components. Specifically, FP-RMS rhabdospheres have increased expression of SOX2, POU5F1 (OCT4), and NANOG, and several receptors and transcriptional regulators in the Notch signaling pathway. FP-RMS rhabdospheres also exhibit functional stemness characteristics including multipotency, increased tumorigenicity in vivo, and chemoresistance. This method provides a novel practical tool to support research into FP-RMS stemness and chemoresistance signaling mechanisms.

SUBMITTER: Slemmons KK 

PROVIDER: S-EPMC7888706 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

A method to culture human alveolar rhabdomyosarcoma cell lines as rhabdospheres demonstrates an enrichment in stemness and Notch signaling.

Slemmons Katherine K KK   Deel Michael D MD   Lin Yi-Tzu YT   Oristian Kristianne M KM   Kuprasertkul Nina N   Genadry Katia C KC   Chen Po-Han PH   Chi Jen-Tsan Ashley JT   Linardic Corinne M CM  

Biology open 20210209 2


The development of three-dimensional cell culture techniques has allowed cancer researchers to study the stemness properties of cancer cells in <i>in vitro</i> culture. However, a method to grow PAX3-FOXO1 fusion-positive rhabdomyosarcoma (FP-RMS), an aggressive soft tissue sarcoma of childhood, has to date not been reported, hampering efforts to identify the dysregulated signaling pathways that underlie FP-RMS stemness. Here, we first examine the expression of canonical stem cell markers in hum  ...[more]

Similar Datasets

2021-02-22 | GSE119716 | GEO
| S-EPMC4035936 | biostudies-literature
| S-EPMC5755394 | biostudies-literature
| S-EPMC8374652 | biostudies-literature
| S-EPMC3071502 | biostudies-literature
| S-EPMC4527315 | biostudies-literature
2012-12-06 | E-GEOD-42750 | biostudies-arrayexpress
| S-EPMC6326011 | biostudies-literature
2012-12-06 | GSE42750 | GEO
| S-EPMC2673763 | biostudies-literature