Ontology highlight
ABSTRACT:
SUBMITTER: Belondrade M
PROVIDER: S-EPMC7893054 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Bélondrade Maxime M Nicot Simon S Mayran Charly C Bruyere-Ostells Lilian L Almela Florian F Di Bari Michele A MA Levavasseur Etienne E Watts Joel C JC Fournier-Wirth Chantal C Lehmann Sylvain S Haïk Stéphane S Nonno Romolo R Bougard Daisy D
Scientific reports 20210218 1
Unlike variant Creutzfeldt-Jakob disease prions, sporadic Creutzfeldt-Jakob disease prions have been shown to be difficult to amplify in vitro by protein misfolding cyclic amplification (PMCA). We assessed PMCA of pathological prion protein (PrP<sup>TSE</sup>) from 14 human sCJD brain samples in 3 substrates: 2 from transgenic mice expressing human prion protein (PrP) with either methionine (M) or valine (V) at position 129, and 1 from bank voles. Brain extracts representing the 5 major clinicop ...[more]