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Supporting data on combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells.

ABSTRACT: Human embryonic stem cells exhibit great potential as a therapeutic tool in regenerative medicine due to their self-renewal and trilineage differentiation capacity. Maintaining this unique cellular state has been shown to rely primarily on the Activin A / TGF? signaling pathway. While most conventional culture media are supplemented with TGF?, in the current study we utilize a modified version of the commercially available mTeSR1, substituting TGF? for Activin A in order to preserve pluripotency. (1) Cells cultured in ActA-mTesR express pluripotency factors NANOG, OCT4 and SOX2 at comparable levels with cells cultured in TGF?-mTeSR. (2) ActA-mTeSR cultured cells retain a physiological karyotype. (3) Cells in ActA-mTeSR maintain their trilineage differentiation capacity as shown in the teratoma formation assay. This system can be used to dissect the role of Activin A, downstream effectors and signaling cascades in human embryonic stem cell responses.

SUBMITTER: Papadopoulos A 

PROVIDER: S-EPMC7893420 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Supporting data on combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells.

Papadopoulos Angelos A   Chalmantzi Varvara V   Hyvönen Marko M   Stellas Dimitris D   Syrrou Marika M   Fotsis Theodore T   Murphy Carol C  

Data in brief 20210206

Human embryonic stem cells exhibit great potential as a therapeutic tool in regenerative medicine due to their self-renewal and trilineage differentiation capacity. Maintaining this unique cellular state has been shown to rely primarily on the Activin A / TGFβ signaling pathway. While most conventional culture media are supplemented with TGFβ, in the current study we utilize a modified version of the commercially available mTeSR1, substituting TGFβ for Activin A in order to preserve pluripotency  ...[more]

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