Ontology highlight
ABSTRACT:
SUBMITTER: Long Y
PROVIDER: S-EPMC7893963 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Genome biology 20210219 1
The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the deve ...[more]