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Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.

ABSTRACT: We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.

SUBMITTER: Hahaut V

PROVIDER: S-EPMC9546769 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.

Hahaut Vincent V Pavlinic Dinko D Carbone Walter W Schuierer Sven S Balmer Pierre P Quinodoz Mathieu M Renner Magdalena M Roma Guglielmo G Cowan Cameron S CS Picelli Simone S

Nature biotechnology 20220530 10

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene ex ...[more]

PMID: 35637419

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