Activity labeling in vivo using CaMPARI2 reveals intrinsic and synaptic differences between neurons with high and low firing rate set points.
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ABSTRACT:
SUBMITTER: Trojanowski NF
PROVIDER: S-EPMC7897300 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Publications
Activity labeling in vivo using CaMPARI2 reveals intrinsic and synaptic differences between neurons with high and low firing rate set points.
Trojanowski Nicholas F NF Bottorff Juliet J Turrigiano Gina G GG
Neuron 20201216 4
Neocortical pyramidal neurons regulate firing around a stable mean firing rate (FR) that can differ by orders of magnitude between neurons, but the factors that determine where individual neurons sit within this broad FR distribution are not understood. To access low- and high-FR neurons for ex vivo analysis, we used Ca<sup>2+</sup>- and UV-dependent photoconversion of CaMPARI2 in vivo to permanently label neurons according to mean FR. CaMPARI2 photoconversion was correlated with immediate early ...[more]
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