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Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer's disease.


ABSTRACT:

Background

High-density oligomers of the prion protein (HDPs) have previously been identified in brain tissues of patients with rapidly progressive Alzheimer's disease (rpAD). The current investigation aims at identifying interacting partners of HDPs in the rpAD brains to unravel the pathological involvement of HDPs in the rapid progression.

Methods

HDPs from the frontal cortex tissues of rpAD brains were isolated using sucrose density gradient centrifugation. Proteins interacting with HDPs were identified by co-immunoprecipitation coupled with mass spectrometry. Further verifications were carried out using proteomic tools, immunoblotting, and confocal laser scanning microscopy.

Results

We identified rpAD-specific HDP-interactors, including the growth arrest specific 2-like 2 protein (G2L2). Intriguingly, rpAD-specific disturbances were found in the localization of G2L2 and its associated proteins i.e., the end binding protein 1, ?-tubulin, and ?-actin.

Discussion

The results show the involvement of HDPs in the destabilization of the neuronal actin/tubulin infrastructure. We consider this disturbance to be a contributing factor for the rapid progression in rpAD.

SUBMITTER: Shafiq M 

PROVIDER: S-EPMC7898440 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer's disease.

Shafiq Mohsin M   Zafar Saima S   Younas Neelam N   Noor Aneeqa A   Puig Berta B   Altmeppen Hermann Clemens HC   Schmitz Matthias M   Matschke Jakob J   Ferrer Isidre I   Glatzel Markus M   Zerr Inga I  

Molecular neurodegeneration 20210222 1


<h4>Background</h4>High-density oligomers of the prion protein (HDPs) have previously been identified in brain tissues of patients with rapidly progressive Alzheimer's disease (rpAD). The current investigation aims at identifying interacting partners of HDPs in the rpAD brains to unravel the pathological involvement of HDPs in the rapid progression.<h4>Methods</h4>HDPs from the frontal cortex tissues of rpAD brains were isolated using sucrose density gradient centrifugation. Proteins interacting  ...[more]

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