Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase.
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ABSTRACT:
SUBMITTER: Kimoto M
PROVIDER: S-EPMC7900958 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase.
Kimoto Michiko M Soh Si Hui Gabriella SHG Tan Hui Pen HP Okamoto Itaru I Hirao Ichiro I
Biopolymers 20201106 1
We present cognate base pair selectivity in template-dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds-Pa. T4 DNA ligase efficiently recognizes the Ds-Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our results indicate that the hydrophobic base pairing is allowed in enzymatic ligation with higher cognate base-pair selectivity, relative to the hydrogen-bond interactions between pairing bases. The efficien ...[more]
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