Ontology highlight
ABSTRACT:
SUBMITTER: Standage-Beier K
PROVIDER: S-EPMC7901017 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Standage-Beier Kylie K Tekel Stefan J SJ Brafman David A DA Wang Xiao X
ACS synthetic biology 20210119 2
CRISPR-based technologies are paramount in genome engineering and synthetic biology. Prime editing (PE) is a technology capable of installing genomic edits without double-stranded DNA breaks (DSBs) or donor DNA. Prime editing guide RNAs (pegRNAs) simultaneously encode both guide and edit template sequences. They are more design intensive than CRISPR single guide RNAs (sgRNAs). As such, application of PE technology is hindered by the limited throughput of manual pegRNA design. To that end, we des ...[more]