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PrimeDesign software for rapid and simplified design of prime editing guide RNAs.

ABSTRACT: Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting >68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.

SUBMITTER: Hsu JY

PROVIDER: S-EPMC7884779 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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PrimeDesign software for rapid and simplified design of prime editing guide RNAs.

Hsu Jonathan Y JY Grünewald Julian J Szalay Regan R Shih Justine J Anzalone Andrew V AV Lam Kin Chung KC Shen Max W MW Petri Karl K Liu David R DR Joung J Keith JK Pinello Luca L

Nature communications 20210215 1

Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and sea ...[more]

PMID: 33589617

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Prime Editing Guide RNA Design Automation Using PINE-CONE.

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| S-EPMC7901017 | biostudies-literature

PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants.

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| S-EPMC7923538 | biostudies-literature

BEtarget: A versatile web-based tool to design guide RNAs for base editing in plants

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Heritable gene editing using FT mobile guide RNAs and DNA viruses.

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| S-EPMC5607865 | biostudies-literature

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