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Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku.

ABSTRACT: Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.

SUBMITTER: Jones CE

PROVIDER: S-EPMC7904001 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Monitoring <i>Schizosaccharomyces pombe</i> genome stress by visualizing end-binding protein Ku.

Jones Chance E CE Forsburg Susan L SL

Biology open 20210215 2

Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize ...[more]

PMID: 33579693

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