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Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability.

ABSTRACT: Phase separation is a major mechanism of macromolecular condensation within cells. A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live fission yeast with 1,6-hexanediol. We find that 1,6-hexanediol causes a drastic decrease in cell survival and growth rate. We also see a reduction in HP1 protein foci and increase in DNA damage foci. However, there is no evidence for increased genomic instability in two classically phase-separated domains, the heterochromatic pericentromere and the nucleolar rDNA repeats. This study reveals that 1,6-hexanediol is a blunt tool for phase separation inhibition and its secondary effects must be taken into consideration during its in vivo use.

SUBMITTER: Jones CE

PROVIDER: S-EPMC10411564 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability.

Jones Chance E CE Forsburg Susan L SL

G3 (Bethesda, Md.) 20230801 8

Phase separation is a major mechanism of macromolecular condensation within cells. A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live fission yeast with 1,6-hexanediol. We find that 1,6-hexanediol causes a drastic decrease in cell survival and growth rate. We also see a reduction in HP1 protein foci and increase in DNA damage foci. However, ...[more]

PMID: 37284815

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