Ontology highlight
ABSTRACT:
SUBMITTER: Heim B
PROVIDER: S-EPMC7904181 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Heim Bastian B Handrick René R Hartmann Marcus D MD Kiefer Hans H
PloS one 20210224 2
Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis, we present a generic approach to assess and optimize refolding yield through thermostability analysis. Since commonly used hydrophobic dyes cannot be applied as probes for membrane protein unfolding, we adapted a technique based on reacting cysteins exposed upon thermal denaturation with fluorescent 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM). Successful expression, purification and refo ...[more]