Dataset Information

Draft genome sequence data of the starch-utilizing yeast Saccharomycopsis fibuligera MBY1320 isolated from Nuruk.

ABSTRACT: Saccharomycopsis fibuligera MBY1320 was isolated from Nuruk, a traditional Korean starter for makgeolli (rice wine) production. This isolate has previously been reported to exhibit thermotolerance and starch-degrading activities. In this data description, we present the draft genome sequence of S. fibuligera MBY1320. The genome contained 19,138,941?bp in 16 contigs, and the internal transcribed spacer region of S. fibuligera MBY1320 rRNA gene showed the highest similarity with that of S. fibuligera NRRL Y-2388. The BioProject sequence has been deposited at DDBJ/EMBL/GenBank and Mendeley database. The GenBank accession numbers are PRJNA598085 for the BioProject data, SAMN13698230 for the BioSample data, and GCA_012062855 for the GenBank data.

SUBMITTER: Park EH

PROVIDER: S-EPMC7905335 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Draft genome sequence data of the starch-utilizing yeast Saccharomycopsis fibuligera MBY1320 isolated from Nuruk.

Park Eun-Hee EH Kim Myoung-Dong MD

Data in brief 20210218

Saccharomycopsis fibuligera MBY1320 was isolated from Nuruk, a traditional Korean starter for makgeolli (rice wine) production. This isolate has previously been reported to exhibit thermotolerance and starch-degrading activities. In this data description, we present the draft genome sequence of S. fibuligera MBY1320. The genome contained 19,138,941 bp in 16 contigs, and the internal transcribed spacer region of S. fibuligera MBY1320 rRNA gene showed the highest similarity wi ...[more]

PMID: 33665273

Similar Datasets

Project description:BACKGROUND:Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. The amylolytic yeast Saccharomycopsis fibuligera has served as a good source of enzymes and genes involved in saccharification. Despite its long history of use in food fermentation and bioethanol production, very little is known about the basic physiology and genomic features of S. fibuligera. RESULTS:We performed whole-genome (WG) de novo sequencing and complete assembly of S. fibuligera KJJ81 and KPH12, two isolates from wheat-based Nuruk in Korea. Intriguingly, the KJJ81 genome (~38 Mb) was revealed as a hybrid between the KPH12 genome (~18 Mb) and another unidentified genome sharing 88.1% nucleotide identity with the KPH12 genome. The seven chromosome pairs of KJJ81 subgenomes exhibit highly conserved synteny, indicating a very recent hybridization event. The phylogeny inferred from WG comparisons showed an early divergence of S. fibuligera before the separation of the CTG and Saccharomycetaceae clades in the subphylum Saccharomycotina. Reconstructed carbon and sulfur metabolic pathways, coupled with RNA-Seq analysis, suggested a marginal Crabtree effect under high glucose and activation of sulfur metabolism toward methionine biosynthesis under sulfur limitation in this yeast. Notably, the lack of sulfate assimilation genes in the S. fibuligera genome reflects a unique phenotype for Saccharomycopsis clades as natural sulfur auxotrophs. Extended gene families, including novel genes involved in saccharification and proteolysis, were identified. Moreover, comparative genome analysis of S. fibuligera ATCC 36309, an isolate from chalky rye bread in Germany, revealed that an interchromosomal translocation occurred in the KPH12 genome before the generation of the KJJ81 hybrid genome. CONCLUSIONS:The completely sequenced S. fibuligera genome with high-quality annotation and RNA-Seq analysis establishes an important foundation for functional inference of S. fibuligera in the degradation of fermentation mash. The gene inventory facilitates the discovery of new genes applicable to the production of novel valuable enzymes and chemicals. Moreover, as the first gapless genome assembly in the genus Saccharomycopsis including members with desirable traits for bioconversion, the unique genomic features of S. fibuligera and its hybrid will provide in-depth insights into fungal genome dynamics as evolutionary adaptation.

Dataset Information

Draft genome sequence data of the starch-utilizing yeast Saccharomycopsis fibuligera MBY1320 isolated from Nuruk.

Publications

Draft genome sequence data of the starch-utilizing yeast <i>Saccharomycopsis fibuligera</i> MBY1320 isolated from Nuruk.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets