Ontology highlight
ABSTRACT:
SUBMITTER: Hankeova S
PROVIDER: S-EPMC7909953 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Hankeova Simona S Salplachta Jakub J Zikmund Tomas T Kavkova Michaela M Van Hul Noémi N Brinek Adam A Smekalova Veronika V Laznovsky Jakub J Dawit Feven F Jaros Josef J Bryja Vítězslav V Lendahl Urban U Ellis Ewa E Nemeth Antal A Fischler Björn B Hannezo Edouard E Kaiser Jozef J Andersson Emma Rachel ER
eLife 20210226
Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: <i>d</i>o<i>u</i>ble resin <i>c</i>asting micro computed <i>t</i>omography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (<i>Jag1<sup>Ndr/Ndr</su ...[more]