Unknown

Dataset Information

0

Human Saliva-Mediated Hydrolysis of Eugenyl-?-D-Glucoside and Fluorescein-di-?-D-Glucoside in In Vivo and In Vitro Models.


ABSTRACT: Eugenyl-?-D-glucopyranoside, also referred to as Citrusin C, is a natural glucoside found among others in cloves, basil and cinnamon plants. Eugenol in a form of free aglycone is used in perfumeries, flavourings, essential oils and in medicinal products. Synthetic Citrusin C was incubated with human saliva in several in vitro models together with substrate-specific enzyme and antibiotics (clindamycin, ciprofloxacin, amoxicillin trihydrate and potassium clavulanate). Citrusin C was detected using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Citrusin C was completely degraded only when incubated with substrate-specific A. niger glucosidase E.C 3.2.1.21 (control sample) and when incubated with human saliva (tested sample). The addition of antibiotics to the above-described experimental setting, stopped Citrusin C degradation, indicating microbiologic origin of hydrolysis observed. Our results demonstrate that Citrusin C is subjected to complete degradation by salivary/oral cavity microorganisms. Extrapolation of our results allows to state that in the human oral cavity, virtually all ?-D-glucosides would follow this type of hydrolysis. Additionally, a new method was developed for an in vivo rapid test of glucosidase activity in the human mouth on the tongue using fluorescein-di-?-D-glucoside as substrate. The results presented in this study serve as a proof of concept for the hypothesis that microbial hydrolysis path of ?-D-glucosides begins immediately in the human mouth and releases the aglycone directly into the gastrointestinal tract.

SUBMITTER: Dziadas M 

PROVIDER: S-EPMC7911702 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Human Saliva-Mediated Hydrolysis of Eugenyl-β-D-Glucoside and Fluorescein-di-β-D-Glucoside in In Vivo and In Vitro Models.

Dziadas Mariusz M   Junka Adam A   Jeleń Henryk H  

Biomolecules 20210127 2


Eugenyl-β-D-glucopyranoside, also referred to as Citrusin C, is a natural glucoside found among others in cloves, basil and cinnamon plants. Eugenol in a form of free aglycone is used in perfumeries, flavourings, essential oils and in medicinal products. Synthetic Citrusin C was incubated with human saliva in several in vitro models together with substrate-specific enzyme and antibiotics (clindamycin, ciprofloxacin, amoxicillin trihydrate and potassium clavulanate). Citrusin C was detected using  ...[more]

Similar Datasets

| S-EPMC1147008 | biostudies-other
| S-EPMC1271230 | biostudies-other
| S-EPMC421613 | biostudies-literature
| S-EPMC6412093 | biostudies-literature
| S-EPMC8369842 | biostudies-literature
| S-EPMC6964290 | biostudies-literature
| PRJNA626402 | ENA
| S-EPMC5876659 | biostudies-literature
| S-EPMC1184925 | biostudies-other
| S-EPMC3116501 | biostudies-literature