Ontology highlight
ABSTRACT: Background
In the urgent campaign to develop therapeutics against SARS-CoV-2, natural products have been an important source of new lead compounds.Results
We herein identified two natural products, ginkgolic acid and anacardic acid, as inhibitors using a high-throughput screen targeting the SARS-CoV-2 papain-like protease (PLpro). Moreover, our study demonstrated that the two hit compounds are dual inhibitors targeting the SARS-CoV-2 3-chymotrypsin-like protease (3CLpro) in addition to PLpro. A mechanism of action study using enzyme kinetics further characterized the two compounds as irreversible inhibitors against both 3CLpro and PLpro. Significantly, both identified compounds inhibit SARS-CoV-2 replication in vitro at nontoxic concentrations.Conclusions
Our finding provides two novel natural products as promising SARS-CoV-2 antivirals.
SUBMITTER: Chen Z
PROVIDER: S-EPMC7914117 | biostudies-literature |
REPOSITORIES: biostudies-literature