Dataset Information

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO₂-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

ABSTRACT: To date, Ag-based nanomaterials have demonstrated a high potential to overcome antibiotic resistance issues. However, bare Ag nanomaterials are prone to agglomeration in the biological environment, which results in a loss of antibacterial activity over time. Furthermore, it is still challenging to collect small-sized Ag nanomaterials right after the synthesis process. In this study, spherical-shaped Ag nanoparticles (NPs) (~6-10 nm) were attached on the surface of cetyltrimethylammonium bromide (CTAB)-loaded mesoporous silica nanoparticles (MSNs) (~100-110 nm). Antibacterial activity tests suggested that the obtained nanocomposite can be used as a highly efficient antibacterial agent against both Gram-negative and Gram-positive bacterial strains. The minimum inhibitory concentration (MIC) recalculated to pure Ag weight in nanocomposite was found to be ~1.84 µg/mL (for Escherichia coli) and ~0.92 µg/mL (for Staphylococcus aureus)-significantly smaller compared to values reported to date. The improved antibacterial activity of the prepared nanocomposite can be attributed to the even distribution of non-aggregated Ag NPs per volume unit and the presence of CTAB in the nanocomposite pores.

SUBMITTER: Abduraimova A

PROVIDER: S-EPMC7917824 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO<sub>2</sub>-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

Abduraimova Aiganym A Molkenova Anara A Duisembekova Assem A Mulikova Tomiris T Kanayeva Damira D Atabaev Timur Sh TS

Nanomaterials (Basel, Switzerland) 20210213 2

To date, Ag-based nanomaterials have demonstrated a high potential to overcome antibiotic resistance issues. However, bare Ag nanomaterials are prone to agglomeration in the biological environment, which results in a loss of antibacterial activity over time. Furthermore, it is still challenging to collect small-sized Ag nanomaterials right after the synthesis process. In this study, spherical-shaped Ag nanoparticles (NPs) (~6-10 nm) were attached on the surface of cetyltrimethylammonium bromide ...[more]

PMID: 33668526

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Project description:The recovery of impaired periodontium is still a challenge to the treatment of periodontitis. This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration. The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration. The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis. Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo. The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 ?g/mL rhAm. Tryptic Soy Broth culture medium was used as a baseline control. Osteogenic effect of rhAm (20 ?g/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 ?g/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media. The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control. In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo. The root slices were treated with rhAm (20 ?g/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 ?g/mL rhAm). The root slices treated with osteogenic medium alone were used as the baseline control. The analyses showed that the mHA/CS particles were 2 ?m in diameter and had a uniform pore size. The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g. The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis. The mHA/CS scaffold sustainably released rhAm. The mHA/CS loaded with 20 ?g/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro. Additionally, it promoted the formation of cementum-like tissue in vivo. Our findings suggest that mHA/CS loaded with 20 ?g/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.

Dataset Information

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO₂-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

Publications

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO<sub>2</sub>-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

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Dataset Information

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO2-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

Publications

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO<sub>2</sub>-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO₂-Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent.