Dataset Information

?-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe₃O₄ and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

ABSTRACT: The utilization of waste-paper-biomass for extraction of important ?-cellulose biopolymer, and modification of extracted ?-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, ?-cellulose fibers were super-magnetized (Fe₃O₄), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (HR-TEM), HR-TEM energy dispersive X-ray spectroscopy (HR-TEM-EDS), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. Laccase immobilized on ?-Cellulose-Fe₃O₄-CTNs (?-Cellulose-Fe₃O₄-CTNs-Laccase) gave significant activity recovery (99.16%) and laccase loading potential (169.36 mg/g). The ?-Cellulose-Fe₃O₄-CTNs-Laccase displayed excellent stabilities for temperature, pH, and storage time. The ?-Cellulose-Fe₃O₄-CTNs-Laccase applied in repeated cycles shown remarkable consistency of activity retention for 10 cycles. After the 10th cycle, ?-Cellulose-Fe₃O₄-CTNs possessed 80.65% relative activity. Furthermore, ?-Cellulose-Fe₃O₄-CTNs-Laccase shown excellent degradation of pharmaceutical contaminant sulfamethoxazole (SMX). The SMX degradation by ?-Cellulose-Fe₃O₄-CTNs-Laccase was found optimum at incubation time (20 h), pH (3), temperatures (30 °C), and shaking conditions (200 rpm). Finally, ?-Cellulose-Fe₃O₄-CTNs-Laccase gave repeated degradation of SMX. Thus, this study presents a novel, waste-derived, highly capable, and super-magnetic nanocomposite for enzyme immobilization applications.

SUBMITTER: Ghodake GS

PROVIDER: S-EPMC7919293 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

Ghodake Gajanan S GS Shinde Surendra K SK Saratale Ganesh D GD Saratale Rijuta G RG Kim Min M Jee Seung-Cheol SC Kim Dae-Young DY Sung Jung-Suk JS Kadam Avinash A AA

Polymers 20210215 4

The utilization of waste-paper-biomass for extraction of important α-cellulose biopolymer, and modification of extracted α-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, α-cellulose fibers were super-magnetized (Fe3O4), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (H ...[more]

PMID: 33672000

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Project description:Extremely high mechanical performance spun bionanocomposite fibers of chitosan (CHI), and cellulose nanofibers (CNFs) were successfully achieved by gel spinning of CHI aqueous viscous formulations filled with CNFs. The microstructural characterization of the fibers by X-ray diffraction revealed the crystallization of the CHI polymer chains into anhydrous chitosan allomorph. The spinning process combining acidic-basic-neutralization-stretching-drying steps allowed obtaining CHI/CNF composite fibers of high crystallinity, with enhanced effect at incorporating the CNFs. Chitosan crystallization seems to be promoted by the presence of cellulose nanofibers, serving as nucleation sites for the growing of CHI crystals. Moreover, the preferential orientation of both CNFs and CHI crystals along the spun fiber direction was revealed in the two-dimensional X-ray diffraction patterns. By increasing the CNF amount up to the optimum concentration of 0.4 wt % in the viscous CHI/CNF collodion, Young's modulus of the spun fibers significantly increased up to 8 GPa. Similarly, the stress at break and the yield stress drastically increased from 115 to 163 MPa, and from 67 to 119 MPa, respectively, by adding only 0.4 wt % of CNFs into a collodion solution containing 4 wt % of chitosan. The toughness of the CHI-based fibers thereby increased from 5 to 9 MJ.m-3. For higher CNFs contents like 0.5 wt %, the high mechanical performance of the CHI/CNF composite fibers was still observed, but with a slight worsening of the mechanical parameters, which may be related to a minor disruption of the CHI matrix hydrogel network constituting the collodion and gel fiber, as precursor state for the dry fiber formation. Finally, the rheological behavior observed for the different CHI/CNF viscous collodions and the obtained structural, thermal and mechanical properties results revealed an optimum matrix/filler compatibility and interface when adding 0.4 wt % of nanofibrillated cellulose (CNF) into 4 wt % CHI formulations, yielding functional bionanocomposite fibers of outstanding mechanical properties.

Dataset Information

?-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe₃O₄ and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

Publications

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe<sub>3</sub>O<sub>4</sub> and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

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OmicsDI is part of the ELIXIR infrastructure

Dataset Information

?-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

Publications

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe<sub>3</sub>O<sub>4</sub> and Thiolated-Chitosan for Efficacious Immobilization of Laccase.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

?-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe₃O₄ and Thiolated-Chitosan for Efficacious Immobilization of Laccase.