Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia.
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ABSTRACT:
SUBMITTER: Ballester V
PROVIDER: S-EPMC7923255 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia.
Ballester Veroushka V Taylor William R WR Slettedahl Seth W SW Mahoney Douglas W DW Yab Tracy C TC Sinicrope Frank A FA Boland Clement R CR Boland Clement R CR Lidgard Graham P GP Cruz-Correa Marcia R MR Smyrk Thomas C TC Boardman Lisa A LA Ahlquist David A DA Kisiel John B JB
Epigenomics 20201222 24
<b>Aim:</b> Acquired molecular changes in Lynch syndrome (LS) colorectal tumors have been largely unstudied. We identified methylated DNA markers (MDMs) for discrimination of colorectal neoplasia in LS and determined if these MDMs were comparably discriminant in sporadic patients. <b>Patients & methods:</b> For LS discovery, we evaluated DNA from 53 colorectal case and control tissues using next generation sequencing. For validation, blinded methylation-specific PCR assays to the selected MDMs w ...[more]
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