Dataset Information

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance.

ABSTRACT:

Background

Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance.

Results

The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1?+?BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56?±?0.61%. The co-culture of TA OE-Vel1?+?BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1?+?BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus.

Conclusion

The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.

SUBMITTER: Karuppiah V

PROVIDER: S-EPMC7927390 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance.

Karuppiah Valliappan V Zhixiang Lu L Liu Hongyi H Vallikkannu Murugappan M Chen Jie J

Microbial cell factories 20210302 1

<h4>Background</h4>Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance.<h4>Results</h4>The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual de ...[more]

PMID: 33653343

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Project description:Microfungal applications are increasing daily in the medical science. Several species of Trichoderma are widely used in agricultural fields as biological control and plant growth promoting agents. The application of Trichoderma asperellum as an entomopathogenic fungus against the Anopheles mosquito, a vector of malaria, is a novel control approach. Controlling malaria with eco-friendly management practices is an urgent need. We isolated three T. asperellum from different natural sources using serial dilution and mosquito baiting techniques. The fungi were identified on the basis of phenotypical and molecular characteristics. The fungi were grown in different natural media to examine spore production ability and the fungal spore suspensions were applied to the anopheline larvae to determine their larvicidal activity in vitro. We investigated the efficacy of crude ME (methanolic extract) and different methanolic fractions (MFs) of the fungal extracts against anopheline larvae. Methanolic Fraction 8 (MF8) exhibited the strongest larvicidal activity. A GC-MS analysis of MF8 and a Chemolibrary search were performed to identify the active agents in the fungal extracts. Among the three isolates of T. asperellum, the TaspSKGN2 isolate showed the lowest LD50 (2.68 × 107 conidia/mL) and LT50 values (12.33 h). The crude ME exhibited LD50 values of 0.073 mg/mL and LT50 values of 11.33 h. MF8 showed LD50 values of 0.059 mg/mL and LT50 values of 8.57 h. In GC-MS study of MF8, 49 compounds were found. Among these, seven compounds (2,3-di hydro thiopene, p-cymene, alpha-pinene, hexadecanoic acid, 8-methyl quinoline, (Z,Z)-9,12-octa decadienoic acid, methyl ester, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-Pyran-4-one-) with high abundance were found to have insecticidal efficacy by a literature survey. We detected a reduction in the phenoloxidase content inside the cuticle and hemolymph of the anopheline larvae after a few hours of interaction with ME (0.073 mg/mL). Thus Trichoderma asperellum has new applications for the control of Anopheles spp. malaria vectors.

Dataset Information

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance.

Background

Results

Conclusion

Publications

Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance.

Similar Datasets

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