Dataset Information

Plasma interleukin-21 levels and genetic variants are associated with susceptibility to rheumatoid arthritis.

ABSTRACT:

Background

Rheumatoid Arthritis (RA) is a chronic inflammatory condition characterized by autoantibodies development and an elevated spectrum of pro-inflammatory cytokines. Previous reports highlighted a relationship between IL-21and the pathogenesis of RA. Although elevated IL-21 levels have been reported in RA patients, the association of common IL-21 genetic variants with a predisposition to RA development in the Chinese population lacks.

Materials and methods

Five hundred and fourteen Chinese subjects (healthy controls: 303 and rheumatoid arthritis patients: 211) were enrolled in the study. Clinical data of patients were collected from medical records, and patients were treated as per the guidelines. Common single nucleotide polymorphisms in the IL-21 gene (rs907715, rs2221903, rs2055979 and rs6822844) were genotyped by TaqMan SNPs genotyping method. IL-21 level in plasma of RA patients and healthy subjects was measured by ELISA.

Results

The plasma level of IL-21 was significantly higher in subjects with rheumatoid arthritis relative to healthy controls (p?ConclusionIL-21 plays a crucial function in rheumatoid arthritis pathogenesis. IL-21 rs2055979 polymorphism is associated with IL-21 plasma levels and is predisposed to RA development in the Chinese population.

SUBMITTER: Hao Y

PROVIDER: S-EPMC7936495 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Plasma interleukin-21 levels and genetic variants are associated with susceptibility to rheumatoid arthritis.

Hao Youguo Y Xie Lijun L Xia Jing J Liu Zhen Z Yang Baoxiu B Zhang Minqin M

BMC musculoskeletal disorders 20210305 1

<h4>Background</h4>Rheumatoid Arthritis (RA) is a chronic inflammatory condition characterized by autoantibodies development and an elevated spectrum of pro-inflammatory cytokines. Previous reports highlighted a relationship between IL-21and the pathogenesis of RA. Although elevated IL-21 levels have been reported in RA patients, the association of common IL-21 genetic variants with a predisposition to RA development in the Chinese population lacks.<h4>Materials and methods</h4>Five hundred and ...[more]

PMID: 33673829

Similar Datasets

Project description:INTRODUCTION: Interleukin (IL)-33 is a proinflammatory cytokine contributing to the pathogenesis of rheumatoid arthritis (RA). The gene encoding IL-33 may serve as a genetic factor and be associated with the risk of RA. To investigate the potential association between IL33 and RA, we performed a case-control study based on Chinese Han population. METHODS: A three-stage case-control study was performed. Two tag single-nucleotide polymorphisms (SNPs) (rs7044343 and rs10975514), mapping to the IL33 gene, were first genotyped in the discovery population. We further genotyped rs7044343 and rs10975514 in the validation and replication population. The associations between the two tag SNPs and phenotypic subgroups of RA and levels of serum IL-33 were assessed with a logistic regression model. RESULTS: In the discovery population, the CC genotype of rs7044343 was associated with RA patients (odds ratio (OR)?=?0.777, 95% confidence interval (CI), 0.611 to 0.988; P?=?0.040). After anti-citrullinated peptide antibody (ACPA) stratification, the CC genotype of rs7044343 was also shown to be a protective genotype in RA without ACPA (OR?=?0.610; 95% CI, 0.379 to 0.982; P?=?0.042). In the validation population and replication population, the association between rs7044343 and RA, especially ACPA-negative RA, was still significant. A meta-analysis of discovery, validation, and replication panels confirmed the association between CC genotype of rs7044343 and RA (Pcombined?=?0.0004; ORcombined?=?0.77; 95% CI, 0.67 to 0.89). No evidence was found for heterogeneity between three sample sets (Phet?=?0.99; I²?=?0%). Similar results were also obtained in ACPA-negative RA (Pcombined?=?0.0002; ORcombined?=?0.57; 95% CI, 0.43 to 0.77). No association was detected between rs10975514 polymorphism and RA susceptibility in the discovery and validation population. The serum levels of IL-33 were significantly lower in the patients with the rs7044343 CC genotype. CONCLUSION: The CC genotype of rs7044343 in IL33 is associated with RA patients and downregulates IL-33 expression in RA.

Dataset Information

Plasma interleukin-21 levels and genetic variants are associated with susceptibility to rheumatoid arthritis.

Background

Materials and methods

Results

Publications

Plasma interleukin-21 levels and genetic variants are associated with susceptibility to rheumatoid arthritis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure