Unknown

Dataset Information

0

MicroRNA expression profile in bovine mammary gland parenchyma infected by coagulase-positive or coagulase-negative staphylococci.


ABSTRACT: MicroRNAs (miRNAs) are short, non-coding RNAs, 21-23 nucleotides in length which are known to regulate biological processes that greatly impact immune system activity. The aim of the study was to compare the miRNA expression in non-infected (H) mammary gland parenchyma samples with that of glands infected with coagulase-positive staphylococci (CoPS) or coagulase-negative staphylococci (CoNS) using next-generation sequencing. The miRNA profile of the parenchyma was found to change during mastitis, with its profile depending on the type of pathogen. Comparing the CoPS and H groups, 256 known and 260 potentially new miRNAs were identified, including 32 that were differentially expressed (p???0.05), of which 27 were upregulated and 5 downregulated. Comparing the CoNS and H groups, 242 known and 171 new unique miRNAs were identified: 10 were upregulated (p???0.05), and 2 downregulated (p???0.05). In addition, comparing CoPS with H and CoNS with H, 5 Kyoto Encyclopedia of Genes and Genomes pathways were identified; in both comparisons, differentially-expressed miRNAs were associated with the bacterial invasion of epithelial cells and focal adhesion pathways. Four gene ontology terms were identified in each comparison, with 2 being common to both immune system processes and signal transduction. Our results indicate that miRNAs, especially miR-99 and miR-182, play an essential role in the epigenetic regulation of a range of cellular processes, including immunological systems bacterial growth in dendritic cells and disease pathogenesis (miR-99), DNA repair and tumor progression (miR-182).

SUBMITTER: Bagnicka E 

PROVIDER: S-EPMC7937231 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

MicroRNA expression profile in bovine mammary gland parenchyma infected by coagulase-positive or coagulase-negative staphylococci.

Bagnicka Emilia E   Kawecka-Grochocka Ewelina E   Pawlina-Tyszko Klaudia K   Zalewska Magdalena M   Kapusta Aleksandra A   Kościuczuk Ewa E   Marczak Sylwester S   Ząbek Tomasz T  

Veterinary research 20210306 1


MicroRNAs (miRNAs) are short, non-coding RNAs, 21-23 nucleotides in length which are known to regulate biological processes that greatly impact immune system activity. The aim of the study was to compare the miRNA expression in non-infected (H) mammary gland parenchyma samples with that of glands infected with coagulase-positive staphylococci (CoPS) or coagulase-negative staphylococci (CoNS) using next-generation sequencing. The miRNA profile of the parenchyma was found to change during mastitis  ...[more]

Similar Datasets

2021-04-14 | GSE172013 | GEO
| S-EPMC4194403 | biostudies-literature
| PRJNA721699 | ENA
| S-EPMC5477815 | biostudies-other
| S-EPMC6429511 | biostudies-literature
| S-EPMC6671284 | biostudies-literature
| S-EPMC8473885 | biostudies-literature
| S-EPMC4744398 | biostudies-literature
2020-07-15 | PXD017768 | Pride
| S-EPMC229192 | biostudies-other