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SIN3A Regulates Porcine Early Embryonic Development by Modulating CCNB1 Expression.


ABSTRACT: SIN3A is the central scaffold protein of the SIN3/histone deacetylase (HDAC) transcriptional repressor complex. SIN3A participates in the mouse preimplantation development by fine-tuning HDAC1 expression. However, it remains unresolved if this functional significance of SIN3A was conserved in other mammals. Herein, RNA-seq results show a large amount of SIN3A mRNA is present in oocytes and early embryos prior to embryonic genome activation and a low amount thereafter, suggesting a maternal origin of SIN3A in pigs, cattle, mice, and humans. Interestingly, immunofluorescence data show that SIN3A protein level peaks at four-cell stage in pigs compared with morula stage in cattle. SIN3A depletion in early embryos causes a developmental arrest at two-cell stage in pigs but does not affect bovine early embryonic development. In contrast with mouse data, SIN3A depletion results in only a slight decrease and even no difference in HDAC1 expression in porcine and bovine early embryos, respectively. In addition, HDAC1 knockdown does not cause two-cell block but leads to a reduced blastocyst rate. By using unbiased RNA-seq approach, we found that Cyclin B1 (CCNB1) transcript level is dramatically reduced. Moreover, CCNB1 knockdown results in a similar phenotype as SIN3A depletion. Injection of exogenous CCNB1 mRNA into SIN3A-depleted embryos could partly rescue embryonic development to pass two-cell stage. In conclusion, our results indicate SIN3A plays an essential role in porcine early embryonic development, which probably involves the regulation of CCNB1 expression.

SUBMITTER: Luo L 

PROVIDER: S-EPMC7937639 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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SIN3A Regulates Porcine Early Embryonic Development by Modulating <i>CCNB1</i> Expression.

Luo Lei L   Dang Yanna Y   Shi Yan Y   Zhao Panpan P   Zhang Yunhai Y   Zhang Kun K  

Frontiers in cell and developmental biology 20210222


SIN3A is the central scaffold protein of the SIN3/histone deacetylase (HDAC) transcriptional repressor complex. SIN3A participates in the mouse preimplantation development by fine-tuning <i>HDAC1</i> expression. However, it remains unresolved if this functional significance of SIN3A was conserved in other mammals. Herein, RNA-seq results show a large amount of <i>SIN3A</i> mRNA is present in oocytes and early embryos prior to embryonic genome activation and a low amount thereafter, suggesting a  ...[more]

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