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SPARC enables genetic manipulation of precise proportions of cells.

ABSTRACT: Many experimental approaches rely on controlling gene expression in select subsets of cells within an individual animal. However, reproducibly targeting transgene expression to specific fractions of a genetically defined cell type is challenging. We developed Sparse Predictive Activity through Recombinase Competition (SPARC), a generalizable toolkit that can express any effector in precise proportions of post-mitotic cells in Drosophila. Using this approach, we demonstrate targeted expression of many effectors in several cell types and apply these tools to calcium imaging of individual neurons and optogenetic manipulation of sparse cell populations in vivo.

SUBMITTER: Isaacman-Beck J

PROVIDER: S-EPMC7939234 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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SPARC enables genetic manipulation of precise proportions of cells.

Isaacman-Beck Jesse J Paik Kristine C KC Wienecke Carl F R CFR Yang Helen H HH Fisher Yvette E YE Wang Irving E IE Ishida Itzel G IG Maimon Gaby G Wilson Rachel I RI Clandinin Thomas R TR

Nature neuroscience 20200720 9

Many experimental approaches rely on controlling gene expression in select subsets of cells within an individual animal. However, reproducibly targeting transgene expression to specific fractions of a genetically defined cell type is challenging. We developed Sparse Predictive Activity through Recombinase Competition (SPARC), a generalizable toolkit that can express any effector in precise proportions of post-mitotic cells in Drosophila. Using this approach, we demonstrate targeted expression of ...[more]

PMID: 32690967

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