Unknown

Dataset Information

0

Superoxide Dismutase 3 Controls the Activation and Differentiation of CD4+T Cells.


ABSTRACT: Superoxide dismutase 3 (SOD3), a well-known antioxidant has been shown to possess immunomodulatory properties through inhibition of T cell differentiation. However, the underlying inhibitory mechanism of SOD3 on T cell differentiation is not well understood. In this study, we investigated the effect of SOD3 on anti-CD3/CD28- or phorbol myristate acetate (PMA) and ionomycin (ION)-mediated activation of mouse naive CD4+ T cells. Our data showed that SOD3 suppressed the expression of activation-induced surface receptor proteins such as CD25, and CD69, and cytokines production. Similarly, SOD3 was found to reduce CD4+T cells proliferation and suppress the activation of downstream pathways such as ERK, p38, and NF-?B. Moreover, naïve CD4+T cells isolated from global SOD3 knock-out mice showed higher expression of CD25, CD69, and CD71, IL-2 production, proliferation, and downstream signals compared to wild-type CD4+T cells. Whereas, the use of DETCA, a known inhibitor of SOD3 activity, found to nullify the inhibitory effect of SOD3 on CD4+T cell activation of both SOD3 KO and wild-type mice. Furthermore, the expression of surface receptor proteins, IL-2 production, and downstream signals were also reduced in Th2 and Th17 differentiated cells upon SOD3 treatment. Overall, our data showed that SOD3 can attenuate CD4+T cell activation through modulation of the downstream signalings and restrict CD4+T cell differentiation. Therefore, SOD3 can be a promising therapeutic for T cell-mediated disorders.

SUBMITTER: Agrahari G 

PROVIDER: S-EPMC7947887 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

altmetric image

Publications

Superoxide Dismutase 3 Controls the Activation and Differentiation of CD4<sup>+</sup>T Cells.

Agrahari Gaurav G   Sah Shyam Kishor SK   Bang Chul Hwan CH   Kim Yeong Ho YH   Kim Tae-Yoon TY  

Frontiers in immunology 20210225


Superoxide dismutase 3 (SOD3), a well-known antioxidant has been shown to possess immunomodulatory properties through inhibition of T cell differentiation. However, the underlying inhibitory mechanism of SOD3 on T cell differentiation is not well understood. In this study, we investigated the effect of SOD3 on anti-CD3/CD28- or phorbol myristate acetate (PMA) and ionomycin (ION)-mediated activation of mouse naive CD4<sup>+</sup> T cells. Our data showed that SOD3 suppressed the expression of act  ...[more]

Similar Datasets

| S-EPMC9436477 | biostudies-literature
| S-EPMC5585019 | biostudies-literature
| S-EPMC8754426 | biostudies-literature
| S-EPMC5179518 | biostudies-literature
2021-12-31 | GSE158237 | GEO
| S-EPMC9616074 | biostudies-literature
| S-EPMC7610646 | biostudies-literature
| S-EPMC6773601 | biostudies-literature
2017-12-05 | GSE106206 | GEO
| S-EPMC3667911 | biostudies-literature