Dataset Information

Au-Ag assembled on silica nanoprobes for visual semiquantitative detection of prostate-specific antigen.

ABSTRACT:

Background

Blood prostate-specific antigen (PSA) levels are widely used as diagnostic biomarkers for prostate cancer. Lateral-flow immunoassay (LFIA)-based PSA detection can overcome the limitations associated with other methods. LFIAbased PSA detection in clinical samples enables prognosis and early diagnosis owing to the use of high-performance signal reporters.

Results

Here, a semiquantitative LFIA platform for PSA detection in blood was developed using Au-Ag nanoparticles (NPs) assembled on silica NPs (SiO2@Au-Ag NPs) that served as signal reporters. Synthesized SiO2@Au-Ag NPs exhibited a high absorbance at a wide wavelength range (400-800 nm), with a high scattering on nitrocellulose membrane test strips. In LFIA, the color intensity of the test line on the test strip differed depending on the PSA concentration (0.30-10.00 ng/mL), and bands for the test line on the test strip could be used as a standard. When clinical samples were assessed using this LFIA, a visual test line with particular color intensity observed on the test strip enabled the early diagnosis and prognosis of patients with prostate cancer based on PSA detection. In addition, the relative standard deviation of reproducibility was 1.41%, indicating high reproducibility, and the signal reporter showed good stability for 10 days.

Conclusion

These characteristics of the signal reporter demonstrated the reliability of the LFIA platform for PSA detection, suggesting potential applications in clinical sample analysis.

SUBMITTER: Kim HM

PROVIDER: S-EPMC7953718 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Publications

Au-Ag assembled on silica nanoprobes for visual semiquantitative detection of prostate-specific antigen.

Kim Hyung-Mo HM Kim Jaehi J An Jaehyun J Bock Sungje S Pham Xuan-Hung XH Huynh Kim-Hung KH Choi Yoonsik Y Hahm Eunil E Song Hobeom H Kim Jung-Won JW Rho Won-Yeop WY Jeong Dae Hong DH Lee Ho-Young HY Lee Sangchul S Jun Bong-Hyun BH

Journal of nanobiotechnology 20210312 1

<h4>Background</h4>Blood prostate-specific antigen (PSA) levels are widely used as diagnostic biomarkers for prostate cancer. Lateral-flow immunoassay (LFIA)-based PSA detection can overcome the limitations associated with other methods. LFIAbased PSA detection in clinical samples enables prognosis and early diagnosis owing to the use of high-performance signal reporters.<h4>Results</h4>Here, a semiquantitative LFIA platform for PSA detection in blood was developed using Au-Ag nanoparticles (NPs ...[more]

PMID: 33712008

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Project description:Compared to inorganic mercury (Hg2+), methyl-mercury (CH3Hg+) and ethyl-mercury (C2H5Hg+) (organic mercury) not only have a much stronger toxicity but also are more easily accumulated by marine organisms to produce bioamplification. Therefore, the simultaneously onsite detection of Hg2+ and organic mercury is of great significance to ensure the safety of seafood, and it is also a hard challenge. We designed a T-rich aptamer, HT7, for specifically recognizing Hg2+ and organic mercury and developed a multicolor aptasensor for simultaneous discrimination and detection of Hg2+ and organic mercury with only bare-eye observation using HT7 as a recognition probe and gold nanorods (AuNRs) as a signal. In the presence of Hg2+ and Ag+, Hg2+ preferentially and specifically bind with HT7 immobilized on AuNRs surface and induce the formation of a monolayer Ag/Hg amalgam on the AuNRs surface after reduction, resulting in a change in color from orange to faint purple and a corresponding shift in the absorption peak from 820 to 730 nm in the solution. However, in the presence of CH3Hg+ or C2H5Hg+ and Ag+, CH3Hg+ or C2H5Hg+ preferentially bind with HT7 immobilized on the AuNRs surface and induce the formation of a monolayer Ag0 on the AuNRs surface after reduction, which results in the change in color from orange to atrovirens and the corresponding shift in the absorption peak shift from 820 to 670 nm in the solution. Thus, the inorganic and organic mercury (total of CH3Hg+ and C2H5Hg+) can be specifically discriminated and detected by only bare-eye observation. The method can be used to simultaneously detect inorganic and organic mercury in seawater by the bare-eye observation with a visual detection limit of 2.0 ppm for Hg2+ and 10.0 ppm for organic mercury. The success of this study is a useful enlightenment to develop an instrument-free method for an onsite detection of trace inorganic and organic mercury in environment by a bare-eye observation, although the sensitivity of the method is relatively low.

Dataset Information

Au-Ag assembled on silica nanoprobes for visual semiquantitative detection of prostate-specific antigen.

Background

Results

Conclusion

Publications

Au-Ag assembled on silica nanoprobes for visual semiquantitative detection of prostate-specific antigen.

Similar Datasets

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