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In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations.

ABSTRACT: Liver and kidney uptake and antisense activity is studied for a series of Locked Nucleic Acid (LNA) oligonucleotides with fully stereo-defined, internucleoside linkages. These stereo-specific phosphorothioates are made with a newly developed synthesis method and are being analyzed both theoretically and experimentally. Their structures are obtained theoretically by using many-body Schrödinger equations applied to a group of 11 stereo-defined LNA antisense oligonucleotides selected for biological experiments. The fully converged electronic structures were obtained from ab initio quantum calculations providing the specific electronic structures. One important result was the observation that the calculated electronic structure, represented by the iso-surface area of the electron density in Å², correlated linearly with LNA oligonucleotide uptake in the liver and kidney. This study also shows that more complex biological phenomena, such as drug activity, will require more molecular and cellular identifiers than used here before a correlation can be found. Establishing biological correlations between quantum mechanical (QM) calculated structures and antisense oligonucleotides is novel, and this method may constitute new tools in drug discovery.

SUBMITTER: Hansen HF

PROVIDER: S-EPMC7973520 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations.

Hansen Henrik Frydenlund HF Albaek Nanna N Hansen Bo Rode BR Shim Irene I Bohr Henrik H Koch Troels T

Scientific reports 20210318 1

Liver and kidney uptake and antisense activity is studied for a series of Locked Nucleic Acid (LNA) oligonucleotides with fully stereo-defined, internucleoside linkages. These stereo-specific phosphorothioates are made with a newly developed synthesis method and are being analyzed both theoretically and experimentally. Their structures are obtained theoretically by using many-body Schrödinger equations applied to a group of 11 stereo-defined LNA antisense oligonucleotides selected for biological ...[more]

PMID: 33737567

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In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations.

Publications

In vivo uptake of antisense oligonucleotide drugs predicted by ab initio quantum mechanical calculations.

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