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A protocol for simultaneous Ca²⁺ and morphology imaging of brain endothelial tip cells in larval zebrafish.

ABSTRACT: Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca²⁺ activities with different spatiotemporal patterns during migration. Examining the Ca²⁺ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca²⁺ activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

SUBMITTER: Liu TT

PROVIDER: S-EPMC7982748 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish.

Liu Ting-Ting TT Hou Han H Du Jiu-Lin JL

STAR protocols 20210318 1

Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ act ...[more]

PMID: 33778782

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