Unknown

Dataset Information

0

A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish.


ABSTRACT: Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

SUBMITTER: Liu TT 

PROVIDER: S-EPMC7982748 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7704940 | biostudies-literature
| S-EPMC10937895 | biostudies-literature
| S-EPMC8042830 | biostudies-literature
| S-EPMC5251937 | biostudies-literature
| S-EPMC9272750 | biostudies-literature
| S-EPMC7290829 | biostudies-literature
| S-EPMC5571186 | biostudies-other
| S-EPMC8383442 | biostudies-literature
| S-EPMC8844900 | biostudies-literature
| S-EPMC6692577 | biostudies-literature