Unknown

Dataset Information

0

Ca2+ dynamics in zebrafish morphogenesis.


ABSTRACT: Intracellular calcium ion (Ca2+) signaling is heavily involved in development, as illustrated by the use of a number of Ca2+ indicators. However, continuous Ca2+ patterns during morphogenesis have not yet been studied using fluorescence resonance energy transfer to track the Ca2+ sensor. In the present study, we monitored Ca2+ levels during zebrafish morphogenesis and differentiation with yellow cameleon, YC2.12. Our results show not only clear changes in Ca2+ levels but also continuous Ca2+ patterns at 24 hpf and later periods for the first time. Serial Ca2+dynamics during early pharyngula period (Prim-5-20; 24-33 hpf) was successfully observed with cameleon, which have not reported anywhere yet. In fact, high Ca2+ level occurred concurrently with hindbrain development in segmentation and pharyngula periods. Ca2+ patterns in the late gastrula through segmentation periods which were obtained with cameleon, were similar to those obtained previously with other Ca2+sensor. Our results suggested that the use of various Ca2+ sensors may lead to novel findings in studies of Ca2+ dynamics. We hope that these results will prove valuable for further research in Ca2+ signaling.

SUBMITTER: Tsuruwaka Y 

PROVIDER: S-EPMC5251937 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6300091 | biostudies-literature
| S-EPMC6107701 | biostudies-literature
| S-EPMC7430993 | biostudies-literature
| S-EPMC7695684 | biostudies-literature
| S-EPMC8421859 | biostudies-literature
| S-EPMC5572320 | biostudies-literature
| S-EPMC6140381 | biostudies-literature
| S-EPMC6581653 | biostudies-literature
| S-EPMC10774910 | biostudies-literature
| S-EPMC7526510 | biostudies-literature