Low-Dose Tamoxifen Induces Significant Bone Formation in Mice.

ABSTRACT: Use of the selective estrogen receptor modulator Tamoxifen (TAM) is a mainstay to induce conditional expression of Cre recombinase in transgenic laboratory mice. To excise β-catenin ^fl/fl in 28-day-old male and female Prrx1-CreER/β-catenin ^fl/fl mice (C57BL/6), we utilized TAM at 150 mg/kg; despite β-catenin knockout in MSC, we found a significant increase in trabecular and cortical bone volume in all genders. Because TAM was similarly anabolic in KO and control mice, we investigated a dose effect on bone formation by treating wild-type mice (WT C57BL/6, 4 weeks) with TAM (total dose 0, 20, 40, 200 mg/kg via four injections). TAM increased bone in a dose-dependent manner analyzed by micro-computed tomography (μCT), which showed that, compared to control,

SUBMITTER: Xie Z 

PROVIDER: S-EPMC7990151 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature