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Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound.

ABSTRACT: Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

SUBMITTER: Xian Q

PROVIDER: S-EPMC7995654 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound.

Xian Quanxiang Q Qiu Zhihai Z Kala Shashwati S Guo Jinghui J Zhu Jiejun J Wong Kin Fung KF Guo Sean Shu Yi SSY Zhu Ting T Hou Xuandi X Sun Lei L

STAR protocols 20210316 2

Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For compl ...[more]

PMID: 33796870

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