Ontology highlight
ABSTRACT:
SUBMITTER: Clark IC
PROVIDER: S-EPMC8000134 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Clark Iain C IC Mensa Bruk B Ochs Christopher J CJ Schmidt Nathan W NW Mravic Marco M Quintana Francisco J FJ DeGrado William F WF Abate Adam R AR
Proceedings of the National Academy of Sciences of the United States of America 20210301 12
The randomization and screening of combinatorial DNA libraries is a powerful technique for understanding sequence-function relationships and optimizing biosynthetic pathways. Although it can be difficult to predict a priori which sequence combinations encode functional units, it is often possible to omit undesired combinations that inflate library size and screening effort. However, defined library generation is difficult when a complex scan through sequence space is needed. To overcome this cha ...[more]