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Effect of low-intensity ultrasound on partial nitrification: Performance, sludge characteristics, and properties of extracellular polymeric substances.

ABSTRACT: Ultrasound technology, which is environment-friendly and economical, has emerged as a novel strategy that can be used to enhance the partial nitrification process. However, its effect on this process remains unclear. Therefore, in this study, partial nitrification sludge was subjected to low-intensity (0.15 W/mL) ultrasound treatment for 10 min, and the effect of ultrasonic treatment on the partial nitrification process was evaluated based on changes in reactor performance, sludge characteristics, and the properties of extracellular polymeric substances (EPS). The results obtained showed that the ultrasonic treatment enhanced nitrite accumulation performance as well as the activity of ammonia-oxidizing bacteria from 3.3 to 16.6 mg O₂/g VSS,⋅while inhibiting the activity of nitrite-oxidizing bacteria. Further analysis showed that owing to the ultrasonic treatment, there was an increase in EPS contents. Particularly, there was a significant increase in loosely bound polysaccharide (PS) contents, indicating the occurrence of intracellular PS anabolics as well as PS secretion. Additionally, ultrasonic treatment induced a significant increase in carbonyl, hydroxyl, and amine functional group contents, and EPS analysis results revealed that it had a positive effect on mass transfer efficiency; thus, it enhanced the partial nitrification process. Overall, this study describes the effect of intermittent low-intensity ultrasound on the partial nitrification process as well as the associated enhancement mechanism.

SUBMITTER: Tian S

PROVIDER: S-EPMC8010210 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundWaste activated sludge (WAS) has recently gained attention as a feedstock for resource recovery. The aim of this study is to investigate the corrosion inhibition efficiencies of extracellular polymeric substances (EPS) extracted from WAS.MethodsThe studied corrosion inhibitors were tested with carbon steel in 3.64% NaCl saturated with CO2 at 25 °C, which is the typical oilfield environment. They were first prepared by EPS extraction (heating at 80 °C), followed by centrifugation for solid and liquid separation, then the supernatant was freeze-thawed five times for sterilization of microorganisms in WAS to terminate metabolic activities in the test inhibitors to ensure consistency in corrosion inhibition. The EPS mixture (supernatant) was then deemed as the test corrosion inhibitor. The inhibition performance was determined using potentiodynamic polarization scans.ResultsWaste activated sludge alone showed unsatisfactory inhibition. However, EPS extracted from WAS showed an optimum inhibition of approximately 80% with 1,000 mg/L of inhibitor. The average total solid (TS) and EPS contents of the WAS were 7,330 mg TS/L WAS and 110 mg EPS/g TS, respectively. Three sets of extracted EPS were scanned with fourier-transform infrared spectroscopy (FTIR) and showed almost overlapping curves, yielding the consistent inhibition performance.DiscussionThe potentiodynamic polarization results indicated that EPS acts as a mixed-type inhibitor which inhibits corrosion on both anode and cathode sites of metal surfaces. Based on the FTIR results, it was assumed that major chemical groups O-H, N-H, C-N, C=O, and C-H contributed to the inhibition by adsorbing on the metal surface, forming a biofilm that acts as a protective barrier to isolate the metal from its corrosive environment. Results show that WAS EPS corrosion inhibitors have inhibition performance comparable to commercial products, signifying their potential in commercialization. This corrosion inhibitor is renewable, biodegradable, non-toxic, and free from heavy metal, making it a superior green corrosion inhibitor candidate. Additionally, turning biomass into value-added product can be beneficial to the environment and, in this case, deriving new materials from WAS could also transform the economics of wastewater treatment operations.

Project description:The aim of this work was to better understand the roles of extracellular polymeric substances (EPS) in membrane biofouling at the single-strain level. In the present study, a total of 23 bacterial strains were isolated from a sludge sample. The EPS extracted from pure-cultured bacteria were assessed for their fouling potentials and were simultaneously analyzed using Fourier transform infrared spectroscopy (FTIR). Further, the impact of calcium on the chemical composition of EPS and membrane fouling behavior was investigated in a strain-dependent manner. The EPS of the 23 bacterial strains exhibited different IR features for protein and polysaccharide regions. In addition, an ?-1,4-glycosidic linkage (920 cm-1) and amide II (1,550 cm-1) correlated very well with the fouling potentials of all pure-cultured bacteria. In contrast to low-fouling strains, medium- and high-fouling strains exhibited two distinct peaks at 1,020 cm-1 (uronic acids) and 1,250 cm-1 (O-acetyl), which accelerate membrane fouling given their gelling capacities. In the presence of calcium, the fouling potential of a high-fouling strain (Bacillus sp. strain JSB10) was profoundly reduced (P < 0.0005) due to the binding activity of an ?-1,4-glycosidic linkage and amide II with calcium. However, the impact of calcium on a low-fouling strain (Vagococcus sp. strain JSB21) was insignificant. Two-dimensional FTIR correlation spectroscopic (2D-FTIR-COS) analysis further revealed that the susceptibilities of functional groups to calcium largely relied on the composition and abundance of the above-described functional groups in EPS. These findings suggest that bacterial strains with different fouling potentials exhibit varied responses to calcium.IMPORTANCE Membrane biofouling is one of the main challenges for the operation of membrane-based processes used for water and wastewater treatment. This study revealed the functional determinants of EPS in membrane biofouling of 23 bacterial strains isolated from a full-scale membrane bioreactor (MBR) plant. We found that an ?-1,4-glycosidic bond, amide II, and uronic acids of EPS significantly correlated with the fouling potentials of bacteria. The roles of these EPS groups in membrane fouling were impacted by calcium resulting from EPS-calcium interactions. In addition, our results also demonstrated that any perturbations in the sludge bacterial community in MBRs can lead to varied filtration potentials of the bulk liquor.

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Effect of low-intensity ultrasound on partial nitrification: Performance, sludge characteristics, and properties of extracellular polymeric substances.

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