High-resolution single-cell transcriptomics reveals heterogeneity of self-renewing hair follicle stem cells.

ABSTRACT: Multipotent bulge stem cells (SCs) fuel the hair follicle (HF) cyclic growth during adult skin homeostasis, but their intrinsic molecular heterogeneity is not well understood. These hair follicle stem cells (HFSCs) engage in bouts of self-renewal, migration and differentiation during the hair cycle. Here, we perform high-resolution single-cell RNA sequencing (scRNA-seq) of HFSCs sorted as CD34⁺ /K14-H2BGFP⁺ from mouse skin at mid-anagen, the self-renewal stage. We dissect the transcriptomic profiles and unravel population-specific transcriptional heterogeneity. Unsupervised clustering reveals five major HFSC populations, which distinguished by known markers associated with both the bulge and the outer root sheath (ORS) underneath. These populations include quiescent b

SUBMITTER: Chovatiya G 

PROVIDER: S-EPMC8016723 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature