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Transient Astrocytic Gq Signaling Underlies Remote Memory Enhancement.


ABSTRACT: Astrocytes elicit transient Ca2+ elevations induced by G protein-coupled receptors (GPCRs), yet their role in vivo remains unknown. To address this, transgenic mice with astrocytic expression of the optogenetic Gq-type GPCR, Optoα1AR, were established, in which transient Ca2+ elevations similar to those in wild type mice were induced by brief blue light illumination. Activation of cortical astrocytes resulted in an adenosine A1 receptor-dependent inhibition of neuronal activity. Moreover, sensory stimulation with astrocytic activation induced long-term depression of sensory evoked response. At the behavioral level, repeated astrocytic activation in the anterior cortex gradually affected novel open field exploratory behavior, and remote memory was enhanced in a novel object recognition task. These effects were blocked by A1 receptor antagonism. Together, we demonstrate that GPCR-triggered Ca2+ elevation in cortical astrocytes has causal impacts on neuronal activity and behavior.

SUBMITTER: Iwai Y 

PROVIDER: S-EPMC8019746 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Transient Astrocytic Gq Signaling Underlies Remote Memory Enhancement.

Iwai Youichi Y   Ozawa Katsuya K   Yahagi Kazuko K   Mishima Tsuneko T   Akther Sonam S   Vo Camilla Trang CT   Lee Ashley Bomin AB   Tanaka Mika M   Itohara Shigeyoshi S   Hirase Hajime H  

Frontiers in neural circuits 20210322


Astrocytes elicit transient Ca<sup>2+</sup> elevations induced by G protein-coupled receptors (GPCRs), yet their role <i>in vivo</i> remains unknown. To address this, transgenic mice with astrocytic expression of the optogenetic Gq-type GPCR, Optoα1AR, were established, in which transient Ca<sup>2+</sup> elevations similar to those in wild type mice were induced by brief blue light illumination. Activation of cortical astrocytes resulted in an adenosine A1 receptor-dependent inhibition of neuron  ...[more]

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