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Quantification of microglial contact and engulfment of oligodendrocyte progenitor cells in the rodent brain.

ABSTRACT: Microglia have emerged as essential regulators of neurodevelopment by phagocytosing synapses. Recently, we showed that microglia engulf viable oligodendrocyte progenitor cells (OPCs) during development to facilitate myelination. Here, we describe a protocol to quantify microglial engulfment of whole cells using 3D confocal microscopy to differentiate microglial contact. This protocol can be applied to assess whole-cell engulfment in a variety of contexts and cell types. For complete details on the use and execution of this protocol, please refer to Nemes-Baran et al. (2020).

SUBMITTER: Nemes-Baran AD

PROVIDER: S-EPMC8025152 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Quantification of microglial contact and engulfment of oligodendrocyte progenitor cells in the rodent brain.

Nemes-Baran Ashley D AD DeSilva Tara M TM

STAR protocols 20210324 2

Microglia have emerged as essential regulators of neurodevelopment by phagocytosing synapses. Recently, we showed that microglia engulf viable oligodendrocyte progenitor cells (OPCs) during development to facilitate myelination. Here, we describe a protocol to quantify microglial engulfment of whole cells using 3D confocal microscopy to differentiate microglial contact. This protocol can be applied to assess whole-cell engulfment in a variety of contexts and cell types. For complete details on t ...[more]

PMID: 33855305

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