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Boronic Acid Functionalized Nanosilica for Binding Guest Molecules.

ABSTRACT: Dendritic fibrous nanosilica (DFNS) has very high surface area and well-defined nanochannels; therefore, it is very useful as supporting material for numerous applications including catalysis, sensing, and bioseparation. Due to the highly restricted space, addition of molecular ligands to DFNS is very challenging. This work studies how ligand conjugation in nanoscale pores in DFNS can be achieved through copper-catalyzed click reaction, using an optional, in situ synthesized, temperature-responsive polymer intermediate. A clickable boronic acid is used as a model to investigate the ligand immobilization and the molecular binding characteristics of the functionalized DFNS. The morphology, composition, nanoscale pores, and specific surface area of the boronic acid functionalized nanosilica were characterized by electron microscopy, thermogravimetric and elemental analysis, Fourier transform infrared spectroscopy, and nitrogen adsorption-desorption measurements. The numbers of boronic acid molecules on the modified DFNS with and without the polymer were determined to be 0.08 and 0.68 mmol of ligand/g of DFNS, respectively. We also studied the binding of small cis-diol molecules in the nanoscale pores of DFNS. The boronic acid modified DFNS with the polymer intermediate exhibits higher binding capacity for Alizarin Red S and nicotinamide adenine dinucleotide than the polymer-free DFNS. The two types of boronic acid modified DFNS can bind small cis-diol molecules in the presence of large glycoproteins, due in large part to the effect of size exclusion provided by the nanochannels in the DFNS.

SUBMITTER: Xue X

PROVIDER: S-EPMC8029584 | biostudies-literature |

REPOSITORIES: biostudies-literature

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