Comparative Effects of Event Detection Methods on the Analysis and Interpretation of Ca²⁺ Imaging Data.

ABSTRACT: Calcium imaging has gained substantial popularity as a tool to profile the activity of multiple simultaneously active cells at high spatiotemporal resolution. Among the diverse approaches to processing of Ca²⁺ imaging data is an often subjective decision of how to quantify baseline fluorescence or F ₀. We examine the effect of popular F ₀ determination methods on the interpretation of neuronal and astrocyte activity in a single dataset of rats trained to self-administer intravenous infusions of cocaine and compare them with an F ₀-independent wavelet ridgewalking event detection approach. We find that the choice of the processing method has a profound impact on the interpretation of widefield imaging results. All of the dF

SUBMITTER: Neugornet A 

PROVIDER: S-EPMC8032960 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature