Dataset Information
BbeaR: an R package and framework for epitope-specific antibody profiling.
Ontology highlight
ABSTRACT: Summary
Analysis of epitope-specific antibody repertoires has provided novel insights into the pathogenesis of inflammatory disorders, especially allergies. A novel multiplex immunoassay, termed Bead-Based Epitope Assay (BBEA), was developed to quantify levels of epitope-specific immunoglobulins, including IgE, IgG, IgA and IgD isotypes. bbeaR is an open-source R package, developed for the BBEA, provides a framework to import, process and normalize .csv data files exported from the Luminex reader, evaluate various quality control metrics, analyze differential epitope-binding antibodies with linear modeling, visualize results and map epitopes' amino acid sequences to their respective primary protein structures. bbeaR enables streamlined and reproducible analysis of epitope-specific antibody profiles.Availability and implementation
bbeaR is open-source and freely available from GitHub as an R package: https://github.com/msuprun/bbeaR; vignettes included.Supplementary information
Supplementary data are available at Bioinformatics online.
SUBMITTER: Suprun M
PROVIDER: S-EPMC8034521 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Publications
bbeaR: an R package and framework for epitope-specific antibody profiling.
Bioinformatics (Oxford, England) 20210401 1
<h4>Summary</h4>Analysis of epitope-specific antibody repertoires has provided novel insights into the pathogenesis of inflammatory disorders, especially allergies. A novel multiplex immunoassay, termed Bead-Based Epitope Assay (BBEA), was developed to quantify levels of epitope-specific immunoglobulins, including IgE, IgG, IgA and IgD isotypes. bbeaR is an open-source R package, developed for the BBEA, provides a framework to import, process and normalize .csv data files exported from the Lumin ...[more]