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Responsive Emulsions for Sequential Multienzyme Cascades.

ABSTRACT: Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side.. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO₂ , pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

SUBMITTER: Sun Z

PROVIDER: S-EPMC8048562 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature

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Responsive Emulsions for Sequential Multienzyme Cascades.

Sun Zhiyong Z Zhao Qingcai Q Haag Rainer R Wu Changzhu C

Angewandte Chemie (International ed. in English) 20210305 15

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side.. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO<sub>2</sub> , pH, and thermo-responsive block copolymer as a stabilizer, all ...[more]

PMID: 33480131

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