Unknown

Dataset Information

0

Responsive Emulsions for Sequential Multienzyme Cascades.


ABSTRACT: Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side.. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2 , pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

SUBMITTER: Sun Z 

PROVIDER: S-EPMC8048562 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5775905 | biostudies-literature
| S-EPMC8457072 | biostudies-literature
| S-EPMC11346303 | biostudies-literature
| S-EPMC3114947 | biostudies-literature
| S-EPMC8772379 | biostudies-literature
| S-EPMC7077715 | biostudies-literature
| S-EPMC8295998 | biostudies-literature
| S-EPMC9350585 | biostudies-literature
| S-EPMC8152521 | biostudies-literature