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An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.


ABSTRACT: The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME and demonstrate that breast cancer cells and macrophages generate hypoxic and nutrient gradients, self-organize, and have spatially varying phenotypes along the gradients, leading to new insights into tumorigenesis.

SUBMITTER: Gilmore AC 

PROVIDER: S-EPMC8050328 | biostudies-literature |

REPOSITORIES: biostudies-literature

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