An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.
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ABSTRACT:
SUBMITTER: Gilmore AC
PROVIDER: S-EPMC8050328 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Publications
An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.
Gilmore Anne C AC Flaherty Sarah J SJ Somasundaram Veena V Scheiblin David A DA Lockett Stephen J SJ Wink David A DA Heinz William F WF
Communications biology 20210415 1
The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME and demonstrate that breast cancer cells and macrophages generate hypoxic and nutrient gradients, self-organize, and have spatially varying phenotypes along the gradients, leading to new insights into ...[more]
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