Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center.

ABSTRACT:

Background

Sonic Hedgehog (Shh) has a catalytic cleft characteristic for zinc metallopeptidases and has significant sequence similarities with some bacterial peptidoglycan metallopeptidases defining a subgroup within the M15A family that, besides having the characteristic zinc coordination motif, can bind two calcium ions. Extracellular matrix (ECM) components in animals include heparan-sulfate proteoglycans, which are analogs of bacterial peptidoglycan and are involved in the extracellular distribution of Shh.

Results

We found that the zinc-coordination center of Shh is required for its association to the ECM as well as for non-cell autonomous signaling. Association with the ECM requires the presence of at least 0.1 μM zinc and is prevented by mutations affecting critical c

SUBMITTER: Jagers C 

PROVIDER: S-EPMC8052667 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature