Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC8053708 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Wang Heming H Huang Rong R Li Ling L Zhu Junjin J Li Zhihong Z Peng Chao C Peng Chao C Zhuang Xuran X Lin Haifan H Shi Shuo S Huang Pengyu P
Cell discovery 20210419 1
High-throughput sequencing reveals the complex landscape of small noncoding RNAs (sRNAs). However, it is limited by requiring 5'-monophosphate and 3'-hydroxyl in RNAs for adapter ligation and hindered by methylated nucleosides that interfere with reverse transcription. Here we develop Cap-Clip acid pyrophosphatase (Cap-Clip), T4 polynucleotide kinase (PNK) and AlkB/AlkB(D135S)-facilitated small ncRNA sequencing (CPA-seq) to detect and quantify sRNAs with terminus multiplicities and nucleoside me ...[more]