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Morphometric and Molecular Diversity among Seven European Isolates of Pratylenchus penetrans.

ABSTRACT: Pratylenchus penetrans is an economically important root-lesion nematode species that affects agronomic and ornamental plants. Understanding its diversity is of paramount importance to develop effective control and management strategies. This study aimed to characterize the morphological and genetic diversity among seven European isolates. An isolate from the USA was included in the molecular analyses for comparative purposes. Morphometrics of the European P. penetrans isolates generally were within the range of the original descriptions for this species. However, multiple morphometric characteristics, including body length, maximum body width, tail length and length of the post-vulval uterine sac showed discrepancies when compared to other populations. Nucleotide sequence-based analyses revealed a high level of intraspecific diversity among the isolates. We observed no correlation between D2-D3 rDNA- and COXI-based phylogenetic similarities and geographic origin. Our phylogenetic analyses including selected GenBank sequences also suggest that the controversy surrounding the distinction between P. penetrans and P. fallax remains.

SUBMITTER: Bogale M

PROVIDER: S-EPMC8066575 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Pratylenchus penetrans is a common and important agricultural pest in Wisconsin, a USA state with a diverse agriculture. We compared populations from around the state to each other and to data published for populations around the world to gain insight on the variability of features important for identification of this cosmopolitan species. Thirteen isolates from samples collected in soybean fields in ten Wisconsin counties were established in monoxenic cultures. Analysis of morphological features revealed the least variable feature for all isolates collectively was vulva percentage. Features less variable within than among isolates were body width, lip region height, and stylet length. Some isolates showed only the smooth tail tip phenotype and others had a mix of smooth and annulated tail phenotypes. A suite of features provided sufficient pattern to group isolates into four clusters according to hierarchical agglomerative clustering and canonical discriminative analyses, but not with enough distinction to be useful for classification. Haplotype analysis based on the COI mitochondrial gene of the 13 cultured isolates, 39 Wisconsin field populations, and published sequences representing five additional USA states and six countries revealed 21 haplotypes, 15 of which occurred in Wisconsin. Ten haplotypes represented in Wisconsin were shared with populations from Europe, South America, Africa, or Asia. Five haplotypes were unique to Wisconsin, six were unique to The Netherlands, and one was unique to Japan suggesting that even more COI diversity will be revealed when more COI sequences for P. penetrans become available. The maximum pairwise sequence variation was 6% and the SNPs did not alter amino acids, indicating cryptic biodiversity within the species worldwide. The cosmopolitan to localized scale of distribution of COI haplotypes could be due to frequent and ongoing dispersal events, facilitated by life history traits and the broad host range of P. penetrans. Regions of diverse agriculture, like Wisconsin, show promise for studying this important pest and our study confirms the utility of the COI mtDNA gene for studying variation within a species.

Project description:Sweet cherry growers are increasingly using semi-dwarfing rootstocks, including the Gisela® series, when replanting orchards. Little is known of the susceptibility of these new cherry rootstocks to Pratylenchus penetrans, a recognized pest of temperate fruit trees worldwide. Two field experiments were planted in 2010, one in the Okanagan Valley of British Columbia and one in the Annapolis Valley of Nova Scotia. Each experiment was a factorial combination of three rootstocks (Gi.3, Gi.5, and Gi.6) × three training systems, with six replicate four-tree plots of each of the nine combinations. Both sites were fumigated prior to planting and population densities of P. penetrans in roots and root-zone soil were subsequently monitored from 2013 through 2017. None of the P. penetrans population parameters (nematodes/kg soil, nematodes/g fine root, and nematodes/kg soil including roots) differed among rootstocks at either site, suggesting that the rootstocks did not differ in their ability to host P. penetrans. At the British Columbia site only there was an inverse relationship between P. penetrans population densities and tree size for Gi.3 trees in four years and for Gi.6 in 2017, suggesting that Gi.3 rootstock is less tolerant than Gi.5 and Gi.6 rootstocks.Sweet cherry growers are increasingly using semi-dwarfing rootstocks, including the Gisela® series, when replanting orchards. Little is known of the susceptibility of these new cherry rootstocks to Pratylenchus penetrans, a recognized pest of temperate fruit trees worldwide. Two field experiments were planted in 2010, one in the Okanagan Valley of British Columbia and one in the Annapolis Valley of Nova Scotia. Each experiment was a factorial combination of three rootstocks (Gi.3, Gi.5, and Gi.6) × three training systems, with six replicate four-tree plots of each of the nine combinations. Both sites were fumigated prior to planting and population densities of P. penetrans in roots and root-zone soil were subsequently monitored from 2013 through 2017. None of the P. penetrans population parameters (nematodes/kg soil, nematodes/g fine root, and nematodes/kg soil including roots) differed among rootstocks at either site, suggesting that the rootstocks did not differ in their ability to host P. penetrans. At the British Columbia site only there was an inverse relationship between P. penetrans population densities and tree size for Gi.3 trees in four years and for Gi.6 in 2017, suggesting that Gi.3 rootstock is less tolerant than Gi.5 and Gi.6 rootstocks.

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Morphometric and Molecular Diversity among Seven European Isolates of Pratylenchus penetrans.

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