Unknown

Dataset Information

0

Altered Neuronal Support and Inflammatory Response in Bipolar Disorder Patient-Derived Astrocytes.


ABSTRACT: Bipolar disorder (BD) is characterized by cyclical mood shifts. Studies indicate that BD patients have a peripheral pro-inflammatory state and alterations in glial populations in the brain. We utilized an in vitro model to study inflammation-related phenotypes of astrocytes derived from induced pluripotent stem cells (iPSCs) generated from BD patients and healthy controls. BD astrocytes showed changes in transcriptome and induced a reduction in neuronal activity when co-cultured with neurons. IL-1β-stimulated BD astrocytes displayed a unique inflammatory gene expression signature and increased secretion of IL-6. Conditioned medium from stimulated BD astrocytes reduced neuronal activity, and this effect was partially blocked by IL-6 inactivating antibody. Our results suggest that BD astrocytes are functionally less supportive of neuronal excitability and this effect is partially mediated by IL-6. We confirmed higher IL-6 in blood in a distinct cohort of BD patients, highlighting the potential role of astrocyte-mediated inflammatory signaling in BD neuropathology.

SUBMITTER: Vadodaria KC 

PROVIDER: S-EPMC8072028 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

2021-03-19 | GSE157509 | GEO
| PRJNA661540 | ENA
| S-EPMC6610155 | biostudies-literature
| S-EPMC8418615 | biostudies-literature
| S-EPMC7198534 | biostudies-literature
| S-EPMC4643621 | biostudies-literature
| S-EPMC8105282 | biostudies-literature
| S-EPMC9809017 | biostudies-literature
| S-EPMC7409972 | biostudies-literature
2022-02-12 | GSE126512 | GEO