Adsorption and Purification of Baicalin from Scutellaria baicalensis Georgi Extract by Ionic Liquids (ILs) Grafted Silica.

ABSTRACT: Baicalin which has multiple biological activities is the main active component of the root of Scutellaria baicalensis Georgi (SBG). Although its isolation and purification by adsorption methods have aroused much interest of the scientific community, it suffered from the poor selectivity of the adsorbents. In this work, an environmentally benign method was developed to prepare ionic liquids (ILs) grafted silica by using IL 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C₄mim]NTf₂) and ethanol as reaction media. The IL 1-propyl-3-methylimidazolium chloride ([C₃mim]Cl) grafted silica ([C₃mim]⁺Cl^-@SiO₂) was used to adsorb and purify baicalin from the root extract of Scutellaria baicalensis Georgi (SBG). Experimental results indicated that the adsorption equilibrium can be quickly achieved (within 10 min). The adsorption behavior of [C₃mim]⁺Cl^-@SiO₂ for baicalin was in good agreement with Langmuir and Freundlich models and the adsorption was a physisorption process as suggested by Dubinin-Radushkevich model. Compared with commercial resins, [C₃mim]⁺Cl^-@SiO₂ showed the strongest adsorption ability and highest selectivity. After desorption and crystallization, a purity of baicalin as high as 96.5% could be obtained. These results indicated that the ILs grafted silica materials were promising adsorbents for the adsorption and purification of baicalin and showed huge potential in the purification of other bioactive compounds from natural sources.

SUBMITTER: Fan Y 

PROVIDER: S-EPMC8073518 | biostudies-literature |

REPOSITORIES: biostudies-literature