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Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex.


ABSTRACT: Here, we present a comprehensive protocol to analyze the roles of disease-related genes in synaptic transmission. We have developed a pipeline of electrophysiological techniques and combined these with optogenetics in the medial prefrontal cortex of mice. This methodology provides a cost-effective, faster, and easier screening approach to elucidate functional aspects of single genes in several regions in the mouse brain such as a specific layer of the mPFC. For complete details on the use and execution of this protocol, please refer to Nagahama et al. (2020) and Sacai et al. (2020).

SUBMITTER: Nagahama K 

PROVIDER: S-EPMC8079664 | biostudies-literature |

REPOSITORIES: biostudies-literature

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