Estimands in epigenome-wide association studies.

ABSTRACT:

Background

In DNA methylation analyses like epigenome-wide association studies, effects in differentially methylated CpG sites are assessed. Two kinds of outcomes can be used for statistical analysis: Beta-values and M-values. M-values follow a normal distribution and help to detect differentially methylated CpG sites. As biological effect measures, differences of M-values are more or less meaningless. Beta-values are of more interest since they can be interpreted directly as differences in percentage of DNA methylation at a given CpG site, but they have poor statistical properties. Different frameworks are proposed for reporting estimands in DNA methylation analysis, relying on Beta-values, M-values, or both.

Results

We present and discuss four possible approaches of achiev

SUBMITTER: Kruppa J 

PROVIDER: S-EPMC8086103 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature