Unknown

Dataset Information

0

PLCγ1 promotes phase separation of T cell signaling components.


ABSTRACT: The T cell receptor (TCR) pathway receives, processes, and amplifies the signal from pathogenic antigens to the activation of T cells. Although major components in this pathway have been identified, the knowledge on how individual components cooperate to effectively transduce signals remains limited. Phase separation emerges as a biophysical principle in organizing signaling molecules into liquid-like condensates. Here, we report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the TCR pathway. PLCγ1 directly cross-links LAT through its two SH2 domains. PLCγ1 also protects LAT from dephosphorylation by the phosphatase CD45 and promotes LAT-dependent ERK activation and SLP76 phosphorylation. Intriguingly, a nonmonotonic effect of PLCγ1 on LAT clustering was discovered. Computer simulations, based on patchy particles, revealed how the cluster size is regulated by protein compositions. Together, these results define a critical function of PLCγ1 in promoting phase separation of the LAT complex and TCR signal transduction.

SUBMITTER: Zeng L 

PROVIDER: S-EPMC8094118 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4892427 | biostudies-literature
| S-EPMC10879784 | biostudies-literature
| S-EPMC10338336 | biostudies-literature
| S-EPMC7959570 | biostudies-literature
| S-EPMC3373411 | biostudies-literature
| S-EPMC7196643 | biostudies-literature
| S-EPMC7321842 | biostudies-literature
| S-EPMC5788022 | biostudies-literature
| S-EPMC4423181 | biostudies-literature
| S-EPMC10589674 | biostudies-literature